E*Colite is a carbohydrate-rich selective medium particularly adept at resuscitation and detection of chlorine stressed coliform and E. coli encountered in drinking, bottled and food/beverage waters. The bag design houses all test components. Simply add water sample– no preparation, no test bottles. The bag also acts as a sample container. There is no need for handing collection bottles or loose sodium thiosulfate tablets. Reagents are stable at room-temperature for up to 12 months.

CHARM® E*Colite TEST (Bag) is U.S. EPA Approved

• Presence/Absence of coliforms and E. coli in potable water
• Unique single-use self-contained, Burst-a-Seal bag
• Thiosulfate and bactericide included
• Coliform results in 28 hours
• Easy interpretation of results – blue color development

E*Colite uses X-gal indicator to produce an indigo blue positive reaction. This blue color, as opposed to traditional yellow color, vastly improves interpretation of turbid, or rust and heterotropic-bacteria contaminated waters.

If the sample turns blue, coliforms are present. If a blue coliform positive sample fluoresces, E. coli are present. If a blue sample does not fluoresce, continue incubating an additional 20 hours (48 hours total). If blue growth fluoresces after the 48 hours, the sample is confirmed for E. coli. If the blue bag does not fluoresce after 48 hours, the sample is coliform positive and E. coli negative.

Sensitive
E*Colite detects a single microorganism in 100 ml water and has been demonstrated to have 98% correlation with reference method LTB/BGLB in detecting 4 log chlorine-stressed coliform. It demonstrates a 93% correlation with reference method LTB/ECMUG in detecting 4 log chlorine-stressed E. coli.

Specific
E*Colite uses selective detergents to minimize non-specific bacteria interferences. It recovers stressed organisms more effectively than LTB/BGLB or ECMUG reference methods. In a 500 ml sample comparability study with 4 log chlorine-stressed coliform, E*Colite recovered 53% positive coliform while the reference method recovered 48%. Stressed E. coli were recovered 57% in E*Colite versus 48% by the reference method.

Bio-safe
A self-enclosed bactericide is provided to introduce a 7-8 log reduction of any grown organisms. The bactericide provides critical bio-safety for operations without access to an autoclave.

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E-Colite Bag Brochure

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